To ensure a long product shelf-life for biopharmaceutical drug products, a stable storage environment is critical. Determination of the optimal environment is initiated early in the development process through preformulation screening. In order to maintain potency and activity of protein active pharmaceutical ingredients (APIs), preservation of structural conformation—a characteristic highly influenced by formulation environment—is essential. However, analytical monitoring of this parameter during preformulation screening is frequently omitted. In this article we describe the use of various biophysical techniques as formulation screening tools, aiding elucidation of protein conformation and protein dynamics. We will also demonstrate the importance of incorporating these techniques in early-stage protein screening studies.