With 20+ years of E. coli-based process development, One 2 One is a world-leading manufacturer of custom, recombinant proteins and peptides.

Process Design

Using a toolbox of proprietary expression sequences and fusion protein technologies that drive high level expression, protEcol™ Services offers:

• Expression construct design
• Integration into vectors
• Plasmid evaluation in cell line (qualified for production of human therapeutics)
• PEGylation to the N-terminus
• C-terminal amidation

Process Development

protEcol™ Services combines scientific expertise and engineering capabilities to develop reproducible, efficient and scalable processes appropriate for large scale manufacture, in accord with GMP. Dedicated teams specialize in the optimization of upstream and downstream processes, analytical development, quality control and assurance.

Scale-up and Technology Transfer

protEcol™ Services currently offers scale-up processes to 500 L capacity. Technology transfer production processes, analytical assays and cleaning methods are qualified, validated and documented. protEcol™ Services can also provide assistance with Chemistry, Manufacturing and Control section submissions.

GMP Manufacture

protEcol™ Services offers cGMP manufacture of API as well as cGMP manufacture and testing of master and working cell banks with long term storage at -80°C. Coordinated through One 2 One centralized management, protEcol™ Services assists with lyophilization development and manufacture and fill/finish services.

Manufactured in Adelaide, Australia