The temperature of chromatographic operations at manufacturing scale which utilize a chilled mobile phase can be difficult to replicate in a scale down model. At the scale of a benchtop ӒKTA Pure^TM 150, small volumes and heat transfer effects may result in material that has come up to ambient temperature prior to entering the chromatography column, even if the buffer or load material is chilled. To counteract this, a countercurrent shell-and-tube heat exchanger was engineered around the column inlet line to chill the mobile phase just prior to entering the chromatography column. The temperature system was measured and characterized for a 1.0 cm x 10.0 cm column of DEAE Sepharose Fast Flow resin. A temperature profile which more closely mimics that of the manufacturing scale was successfully obtained in this study, confirming the applicability of the shell-and-tube heat exchanger for the scale down model.

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