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Solid phase peptide synthesis (SPPS) is a technique for peptide synthesis.
Released By Bachem
June 28, 2023
Solid phase peptide synthesis (SPPS) is a technique for peptide synthesis. It can be defined as a process in which a peptide anchored by its C-terminus to an insoluble polymer is assembled by the successive addition of the protected amino acids constituting its sequence. Each amino acid addition is referred to as a cycle consisting of: a) cleavage of the Nα-protecting group b) washing steps c) coupling of a protected amino acid d) washing steps As the growing chain is bound to an insoluble support the excess of reagents and soluble by-products can be removed by simple filtration. Washing steps with appropriate solvents ensure the complete removal of cleavage agents after the deprotection step as well as the elimination of excesses of reagents and by-products resulting from the coupling step. For a general scheme of SPPS see below:
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