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Last Updated Friday, July 25 2014
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High Pressure Liquid Chromatography (HPLC)



A separation technique based on a solid stationary phase and a liquid mobile phase. Separations (into distinct bands) are achieved by partition, adsorption, or ion-exchange processes, depending upon the type of stationary phase used. Each band is then profiled as the solvent flows through a UV detector, or by fluorescence, or refractive index detectors. Sometimes called high-performance liquid chromatography.


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