Agilent Expands HPLC Column Portfolio to Enhance Biotherapeutic Analysis

Editor’s Take: Streamlining workflows is essential to facilitating applications such as aggregate characterization, molecular weight confirmation, and size distribution analysis.

Agilent Technologies is expanding its Altura high-performance liquid chromatography (HPLC) portfolio by introducing two new columns. Both columns are now available globally.

Agilent’s Columns Explained

The size-exclusion chromatography (SEC) and polymeric reversed-phase (PLRP-S) columns support critical analytical workflows in biopharmaceutical development and production.

Among the biotherapeutic modalities bolstered by this column technology are peptides, proteins, oligonucleotides, and conjugated therapeutics. Glucagon-like peptide-1 (GLP-1) drugs, monoclonal antibodies, and messenger RNA (mRNA) therapeutics can be found among these groupings.

Analytical column technologies remain essential for applications like aggregate characterization, molecular weight confirmation, and size distribution analysis. These help evaluate product quality, stability, and performance.

At the same time, determining drug-to-antibody ratio is critical for characterizing antibody-drug conjugates as modalities’ complexity continues to increase.

Importance of HPLC Precision

For aggregate analysis of the popular GLP-1s specifically, Agilent says its SEC columns provide twice the sensitivity of inert competitors.

Meanwhile, Agilent says the PLRP-S columns deliver robust performance within demanding conditions of liquid chromatography—mass spectrometry (LC–MS) workflows.

The Altura line of columns falls under Agilent’s broader portfolio of inert liquid chromatography solutions.

David Edwards, Vice President and General Manager of Agilent’s Chemistries and Supplies Division, summarized the response thus far.

“Reception of the first wave of Altura columns has been overwhelmingly positive, highlighting strong demand for high-performance solutions,” Edwards said. “We are expanding access to inert separation technologies that help scientists achieve more reliable, accurate and reproducible results across workflows.”